Effect of deregulation of repressor-specific carbon catabolite repression on carbon source consumption in Escherichia coli

Abstract Escherichia coli has been used as a host to construct the cell factory for biobased production of chemicals from renewable feedstocks. Because galactose is found in marine biomass major component, strategy utilization E. gained more attention. Although and glucose co-fermentation reported using engineered strain, few reports have covered fermentation supplemented with sole carbon source mutant lacking repressor-specific catabolite repression (CCR). Here, we report effects deregulation CCR ( galR − galS ) source, strains. In double (GR2 strain), an increase rates sugar consumption growth was observed compared parent strain. fermentation, wild-type W3110 its GR2 GR2PZ , pfkA zwf consumed at higher rate than those values obtained fermentation. However, GR2P strain showed no difference between fermentations source. This study provides essential information CCR-deregulated